Mutation Category — ALL (required if SINGLE not provided)
Expected format: Missense;Nonsense;Silent Priority logic: Nonsense or Splice-site wins if present → then Missense → then Silent. Silent is only assigned when it is the only mutation type for that guide.
Mutation Category — SINGLE (optional, takes priority over ALL)
Only select this if you already have a clean, single-value mutation category column in your Excel sheet (e.g. "Missense", "Nonsense"). Leave blank otherwise.
Gene Name (optional)
Used to group guides by gene/target. Guides whose Gene Name contains "control", "NTC", "non-target", or similar are automatically grouped as Controls (NTC) and plotted separately.
high_in_treatment (optional - MAGeCK)
Step 2: Configure statistics
Add statistics with names and columns
Step 3: Configure proteins & domains
How to add a protein:
(1) Pick a gene from your data.
(2) Choose Library (preset proteins with domains) or Custom (your own sequence).
(3) Click Add Protein — it appears in the list below.
Repeat for each gene you want to analyze, then click Load Data.
Step 1: Select Gene
Pick a gene; the source options will appear below.
Step 2: Choose Protein Source
Gradient color scheme for heatmap
Details
View Hits on 3D Structure
Residues passing your cutoffs are highlighted on the PDB structure. If your screen residue numbers differ from PDB numbering, adjust the offset.
PDB ID (from RCSB)
— or — Upload own .pdb file
Chain
Manual offset (fallback only)
Color by:
Style:
Background:
BG opacity:1.0
🧬
Enter a PDB ID and click Load Structure
Step 2: Map Genome Screen Columns
Map your Excel columns to the required fields. Gene Name is required. Auto-detection is applied based on column names.
Gene Name *
Guide ID (optional)
Score (optional — e.g. enrichment score, IGTIOB)
Log₂(Fold change)
Transform:
P-value
Format:
FDR / Adjusted P-value
Format:
High in Treatment (optional)
Total Insertions (optional)
EditScreen | Genome-Wide CRISPR Screen
Total Genes
—
Above cutoff
—
Screen:
Hit marker:
Shape
Color
Size9
Size byminmax
Opacity0.85
Tip: click points to pin gene namesFind gene
Axis range:
Filtered gene list · GO button opens enrichment tools (Enrichr, g:Profiler, STRING)
EditScreen | Genome Comparison
Screen A Cutoffs
Score
Log₂(Fold change)
P-value
FDR
Screen B Cutoffs
Score
Log₂(Fold change)
P-value
FDR
X Axis
Y Axis
Graph Font
Font Size
Symbol size:9
Opacity:0.85
Size byminmax
Tip: click points to pin gene namesFind gene
Screen A:ShapeColor
Screen B:ShapeColor
Common:ShapeColor
Show:
X-axis (Screen A)
Y-axis (Screen B)
Screen A Only
Screen B Only
Common Hits
Overlay/Correlation downloads include per-screen values split into categories (Common / A only / B only / Below cutoff) ready for Prism.
🔬 GO / Pathway Analysis
Submit your hit genes to external enrichment tools.
Enrichr — GO, KEGG, Reactome (submits automatically) |
g:Profiler — opens tool; paste your gene list if not pre-filled |
STRING — protein network (max 400 genes)
EditScreen | Multi-Screen Comparison
Significance Cutoffs
Screen A
Z-score
Log₂(Fold change)
P-value
FDR
High in Treatment
Screen B
Z-score
Log₂(Fold change)
P-value
FDR
High in Treatment
Show:
Axis range:
Screen A Only
Screen B Only
Common Guides
All hit guides (passing cutoffs) with full statistics for both screens